• 2018-07
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  • br Ubiquitin Capture by the


    Ubiquitin Capture by the Mobile APC11 RING Domain Contributes to Processive Substrate Ubiquitylation Although the rules of Ub-mediated proteolysis are only beginning to emerge, the rate and order in which different APC/C substrates are degraded during the Sotalol synthesis correlates with processivity of their ubiquitylation. Highly processive substrates receive enough Ubs in a single binding event to enable proteasome binding, and are apparently degraded earlier during the cell cycle than nonprocessive substrates that must cycle on and off APC/C numerous times to receive enough Ubs for proteasomal targeting [71]. Degradation of less-processive substrates can be further slowed by competition with other substrates preventing their rebinding to APC/C, and deubiquitylation removing the few initially linked Ubs [72]. Processivity is determined in part by the rate of dissociation of a substrate from APC/C, which depends on the affinity and arrangement of degron sequences. In addition, a substrate evolves during ubiquitylation, which affects processivity as revealed by recent single-molecule experiments. In a process called processive affinity amplification, ubiquitylation increases the duration of a substrate on APC/C and propensity for further ubiquitylation [73]. Processive affinity amplification is determined in part by mobile elements within the APC/C catalytic core, whereby a substrate-linked Ub binds an unprecedented Ub-binding site that is distinct from the UBE2C–Ub binding site on the RING domain of APC11 [74]. The RING domain simultaneously captures a Ub linked to a substrate and places UBE2C to ubiquitylate another site on a substrate [50]. Accordingly, mutationally eliminating the secondary RING–ubiquitin interaction decreases processivity in vitro, reducing the number of Ubs received by a given substrate molecule, while increasing the number of substrates receiving at least one Ub [50], although other Ub-binding sites within APC/C that contribute to processive affinity amplification or other functions remain to be described.
    APC/C Uses a Dynamic Cullin–RING Mechanism to Elongate Polyubiquitin Chains Human APC/C generates Lys11-linked poly-Ub chains through an entirely different mechanism, via the distinctive E2 enzyme, UBE2S 30, 31, 32. Although APC2 and APC11 are necessary and sufficient to activate UBE2S, the mechanism was not overtly obvious because UBE2S lacks the hallmark E2 residues known to engage RING domains [31]. Indeed, mutation of the APC11 residues binding UBE2C do not impair UBE2S-mediated polyubiquitylation 74, 75. Furthermore, unlike the reaction with UBE2C that is stimulated by coactivator, the intrinsic Ub chain building activity of UBE2S (i.e., transfer of a donor Ub onto a free acceptor Ub) is activated by recombinant APC/C that can be prepared independently of coactivator 21, 74. Instead, a primary function of APC/C is recruiting and positioning the acceptor Ub for its Lys11 to accept another Ub from UBE2S 74, 75.